Human Regulatory T Cell Cocktail Mouse anti-Human, Alexa Fluor 647, FITC, PE-Cy7, Clone: hIL-7R-M21, 2A3, SK3, BD

Catalog No.	Quantity
BDB560249	50 Tests

Catalog No. BDB560249 Supplier BD Biosciences Supplier No. 560249

Description

Mouse Monoclonal Antibody

The Human Regulatory T cell cocktail is a three color reagent used to identify the natural T regulatory cell (nTreg) population. An expression pattern of CD4+CD25int/brightCD127dim closely correlates to the expression of the transcription factor Forkhead box P3 (FoxP3). FoxP3 is considered a specific marker to T regulatory cells (Tregs). The SK3 antibody recognizes CD4, an antigen expressed primarily on helper/inducer T lymphocytes, although this antigen is also present in low density on monocytes. The 2A3 antibody recognizes CD25, an antigen also known as the human low-affinity interleukin-2 receptor (IL-2R). The CD25 antigen is present on a subset of peripheral blood lymphocytes. Antigen density increases on phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes; T lymphocytes from mixed lymphocyte cultures; and human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines. hIL-7R-M21 reacts with the 60 - 90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα). The receptor is a heterodimer composed of the CD127 and the common gamma chain, shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, and IL-15R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Cocktail components: FITC anti-Human CD4, PE-Cy7 anti-Human CD25, Alexa Fluor™ 647 anti-Human CD127
Clone: SK3, 2A3, hIL-7R-M21
IsoType: Isotype: Mouse IgG1, κ
Format: Isotype: FITC, PE-Cy™7, Alexa Fluor™ 647

Flow Cytometry

Specifications

• Antigen: Human Regulatory T Cell Cocktail
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: hIL-7R-M21, 2A3, SK3
• Conjugate: Alexa Fluor 647/FITC/PE-Cyanine7
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1