Gibco™ Human ROR1 Recombinant Protein, PeproTech®

Description

Recombinant Protein

PeproTech's Recombinant Human ROR1 is a glycoprotein containing 377 amino acid residues, and has a calculated molecular weight of approximately 42.4 kDa. As a result of glycosylation, Recombinant Human ROR1 migrates with an apparent molecular mass of approximately 55-65 kDa by SDS-PAGE gel, under reducing and non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated, surface protein predominantly expressed during embryogenesis, where it is involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival. Virtually absent from normal pediatric and adult tissues, with the exception of low-level expression in a subset of immature b cell precursors known as hematogones and adipocytes, ROR1 is notably overexpressed, and considered a survival factor, in a number of B lymphoid and epithelial malignancies: including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, lung adenocarcinoma. First identified during PCR-based cloning of a human neuroblastoma cell line in search of tyrosine kinases similar to tropomyosin-receptor-kinase (Trk) neurotropic receptors, ROR1, along with the related receptor tyrosine kinase (RTK) ROR2, was catalogued as an 'orphan' receptor due to the fact its related ligand remained elusive. Wnt-5a has since been suggested as a candidate ligand for ROR1, and ROR1 has been implicated to function as a pseudokinase, promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a, and TCL1-co-activation of AKT. ROR1 is expressed as a glycoprotein containing extracellular immunoglobulin (Ig)-like, Frizzled, and Kringle domains, as well as an intracellular region containing a tyrosine kinase domain.

Specifications

• Accession Number: Q01973
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 4919
• Molecular Weight (g/mol): 42.4 kDa
• Name: Human ROR1
• Quantity: 100 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: 2810404D04Rik; dJ537F10.1; Inactive tyrosine-protein kinase transmembrane receptor ROR1; MGC99659; mRor1; neurotrophic tyrosine kinase, receptor related 1; neurotrophic tyrosine kinase, receptor-related 1; Ntrkr1; receptor tyrosine kinase like orphan receptor 1; receptor tyrosine kinase-like orphan receptor 1; RGD1559469; ROR1; T-ROR1; tyrosine-protein kinase transmembrane receptor ROR1
• Common Name: ROR1
• Gene Symbol: ROR1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: QETELSVSAE LVPTSSWNIS SELNKDSYLT LDEPMNNITT SLGQTAELHC KVSGNPPPTI RWFKNDAPVV QEPRRLSFRS TIYGSRLRIR NLDTTDTGYF QCVATNGKEV VSSTGVLFVK FGPPPTASPG YSDEYEEDGF CQPYRGIACA RFIGNRTVYM ESLHMQGEIE NQITAAFTMI GTSSHLSDKC SQFAIPSLCH YAFPYCDETS SVPKPRDLCR DECEILENVL CQTEYIFARS NPMILMRLKL PNCEDLPQPE SPEAANCIRI GIPMADPINK NHKCYNSTGV DYRGTVSVTK SGRQCQPWNS QYPHTHTFTA LRFPELNGGH SYCRNPGNQK EAPWCFTLDE NFKSDLCDIP ACDSKDSKEK NKMEILY
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.