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Human Semaphorin 3A Antibody, R&D Systems™
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Catalog No. AF1250SP
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AF1250SP 25 μg
AF1250 100 μg
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Goat Polyclonal Antibody

Semaphorin 3A Polyclonal specifically detects Semaphorin 3A in Human samples. It is validated for Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

Antigen Semaphorin 3A
Applications Western Blot, Flow Cytometry, CyTOF
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q14563
Gene Alias (semaphorin) 3A, coll-1, collapsin 1, Hsema-I, Hsema-III, MGC133243, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema III, SEMA1, Sema3A, SEMAD, SEMAIII, SEMAL, semaphorin D, Semaphorin III, semaphorin L, semaphorin-3A, SemD
Gene Symbols SEMA3A
Host Species Goat
Immunogen Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A Lys26-Val771 (Arg552Ala, Arg555Ala) Accession # Q14563
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 10371
Test Specificity Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3B, rhSemaphorin 3C, rhSemaphorin 3D, rhSemaphorin 3E and rhSemaphorin 3F is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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