Invitrogen™ FasL (Soluble) Human ELISA Kit

For the quantitative detection of human sFas-L.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sFas-L
Biotin-Conjugate anti-human sFas-L monoclonal antibody
Streptavidin-HRP
Human sFas-L Standard lyophilized, 20ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Catalog Number	Mfr. No.	Quantity
50-181-85	Invitrogen™ BMS2602	96 Tests
50-181-87	Invitrogen™ BMS2602TEN	10 x 96 Tests

Description

The Human Fas Ligand (Hu FasL) ELISA quantitates Hu FasL in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FasL. Principle of the method The Human FasL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P48023
• Assay Sensitivity: 0.07 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ALPS1B,APT1LG1,APTL,CD178,CD95-L,CD95L,FASL,TNFSF6,TNLG1A
• Intraassay CV: 6.1%
• Label or Dye: HRP
• Sample Volume: Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: CD178, CD95L protein, Apoptosis antigen ligand
• Test Time: 1 hr. 20 min.

• Assay Range: 0.16-10.0 ng/mL
• Conjugate: Biotin
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 356
• Interassay CV: 7%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

For Research Use Only