Invitrogen™ Human sFRP-1 ELISA Kit

Description

ELISA

Human sFRP-1 quantitates human sFRP-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human sFRP-1.

Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Specifications

• Accession Number: 2210415K03Rik; AW011917; AW107218; AW742929; frizzled-related protein; FRP; FRP1; FRP-1; FrzA; SARP2; SARP-2; Secreted apoptosis-related protein 2; secreted frizzled related protein 1; secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; Sfrp1; sFRP-1
• Assay Range: 0.78 to 50 ng/mL
• Assay Sensitivity: 0.78 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6422
• Gene Symbol: SFRP1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: SFRP1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov