Gibco™ Human IL-12 Receptor beta-1 (soluble) Fc Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human sIL-12 Receptor Beta-1 Fc has a calculated molecular weight of 84.3 kDa and contains 758 amino acid residues, which consists of the extracellular domain of IL-12R Beta-1 fused to the Fc portion of human IgG and contains a C-terminal His-tag. Due to glycosylation, it migrates at an approximate molecular weight of 116 kDa, under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukin 12 with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Specifications

• Accession Number: P42701
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 3594
• Molecular Weight (g/mol): 84.3 kDa
• Name: Human IL-12 Receptor beta-1 (soluble) Fc
• Purification Method: HPLC, SDS-PAGE
• Quantity: 1 mg
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: CD212; cluster of differentiation 212; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R; IL-12 R subunit beta-1; IL-12 R[b]; IL12RB; Il12rb1; IL-12 RB1; IL-12 R-BETA1; IL-12 R-beta-1; IMD30; interleukin 12 receptor subunit beta 1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; Interleukin-12 receptor beta-1 chain precursor (IL-12 R-beta1) (Interleukin-12 receptor beta) (IL-12 receptor beta component); interleukin-12 receptor subunit beta-1; MGC34454
• Common Name: IL12RB1
• Gene Symbol: IL12RB1
• Biological Activity: Human IL-12 Receptor β-1 Fc activity is measured by its ability to bind to Human IL-12p70 in a functional ELISA.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag, His-tag
• Sequence: CRTSECCFQD PPYPDADSGS ASGPRDLRCY RISSDRYECS WQYEGPTAGV SHFLRCCLSS GRCCYFAAGS ATRLQFSDQA GVSVLYTVTL WVESWARNQT EKSPEVTLQL YNSVKYEPPL GDIKVSKLAG QLRMEWETPD NQVGAEVQFR HRTPSSPWKL GDCGPQDDDT ESCLCPLEMN VAQEFQLRRR QLGSQGSSWS KWSSPVCVPP ENPPQPQVRF SVEQLGQDGR RRLTLKEQPT QLELPEGCQG LAPGTEVTYR LQLHMLSCPC KAKATRTLHL GKMPYLSGAA YNVAVISSNQ FGPGLNQTWH IPADTHTEPV ALNISVGTNG TTMYWPARAQ SMTYCIEWQP VGQDGGLATC SLTAPQDPDP AGMATYSWSR ESGAMGQEKC YYITIFASAH PEKLTLWSTV LSTYHFGGNA SAAGTPHHVS VKNHSLDSVS VDWAPSLLST CPGVLKEYVV RCRDEDSKQV SEHPVQPTET QVTLSGLRAG VAYTVQVRAD TAWLRGVWSQ PQRFSIEGGP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHHHH
• Content And Storage: -20°C
• Activity: Human IL-12 Receptor β-1 Fc activity is measured by its ability to bind to Human IL-12p70 in a functional ELISA.
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥98% by SDS-PAGE gel and HPLC analyses.