Human SIRP alpha/CD172a Antibody, R&D Systems™

Mouse Monoclonal Antibody

Supplier:  R&D Systems MAB4546

Catalog No. MAB4546

Description

SIRP alpha/CD172a Monoclonal specifically detects SIRP alpha/CD172a in Human samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: SIRP alpha/CD172a
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 2 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
• Gene Accession No.: P78324
• Gene Symbols: SIRPA
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha/CD172a Gly27-Asn370 (predicted) Accession # P78324
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human SIRP alpha/CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed.
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF
• Clone: 602411
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: Bit, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD1, MYD-1, MyD-1 antigen, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRPA, SIRPalpha, SIRP-ALPHA-1, Sirp-alpha-2, SIRPalpha2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 140885
• Reconstitution: Sterile PBS to a final concentration of 0.5 mg/mL.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2b