Human SPARC Antibody, R&D Systems™

Goat Polyclonal Antibody has been used in 11 publications

Supplier:  R&D Systems AF941SP

Catalog No. AF941SP

Description

SPARC Polyclonal specifically detects SPARC in Human samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

• Antigen: SPARC
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: Basement-membrane protein 40, BM-40, ONcysteine-rich protein, osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin)
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 6678
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 2 ug/mL, Simple Western 20 ug/mL, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
• Gene Accession No.: P09486
• Gene Symbols: SPARC
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SPARC/Osteonectin Ala18-Ile303 Accession # P09486
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SPARC is observed, and less than 3% cross-reactivity with recombinant human SPARC L1 is observed.
• Target Species: Human
• Isotype: IgG

For Research Use Only