Invitrogen™ Human SCF Recombinant Protein

Description

Recombinant Protein

For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Reconstitute lyophilized material in 0.1 mL of 10 mM Acetic acid to a final concentration of 0.1 mg/mL. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C. Avoid multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Specifications

• Accession Number: P21583
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: 0.01 M acetic acid with no preservative
• Gene ID (Entrez): 4254
• Molecular Weight (g/mol): 18.4 kDa
• Name: Human SCF
• Quantity: 10 μg
• Source: E. Coli
• Regulatory Status: RUO
• Gene Alias: 710-712; blz; c-Kit ligand; Clo; cloud gray; Con; contrasted; CSF; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; Gb; grizzle-belly; hematopoietic growth factor KL; H-SCF; Kit ligand; Kitl; Kitlg; kit-ligand; KL-1; Mast cell growth factor; MGF; M-SCF; Processed kit ligand; SCF; SF; SHEP7; sKITLG; Sl; SLF; Soluble KIT ligand; Steel factor; steel factor/kit ligand; stem cell factor; stem cell factor KL-1; stem cell factor, CSF
• Common Name: SCF
• Gene Symbol: KITLG
• Biological Activity: ED50 < 2 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov