Invitrogen™ Human Tankyrase (aa 80-145) Control Fragment Recombinant Protein

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (62%), Rat (62%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287). Stimulates 26S proteasome activity (PubMed:23622245). [UniProt]

Specifications

• Accession Number: O95271
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 8658
• Name: Human Tankyrase (aa 80-145) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 4930554K12Rik; ADP-ribosyltransferase diphtheria toxin-like 5; AI662855; ARTD5; C86528; D130072O21Rik; HGNC:11941; LOW QUALITY PROTEIN: tankyrase-1; mTNKS1; PARP5A; PARP-5 A; PARPL; pART5; Poly [ADP-ribose] polymerase 5 A; Poly [ADP-ribose] polymerase tankyrase-1; poly [ADP-ribose] polymerase tankyrase-1; tankyrase-1; Protein poly-ADP-ribosyltransferase tankyrase-1; TANK1; tankyrase; tankyrase 1; tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; tankyrase-1; TIN1; TINF1; TNKS; TNKS1; TNKS-1; TRF1-interacting ankyrin-related ADP-ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase 1
• Common Name: Tankyrase
• Gene Symbol: TNKS
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: SPDPVDGTSCCSTTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining