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Invitrogen™ Human Tau pT181 ProQuantum Immunoassay Kit

Catalog No. A467395
Encompass_Preferred
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Quantity:
5 x 96 Tests
96 Tests

ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

  • High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
  • Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
  • Fast, easy workflow-2 hours from sample to answer, with no wash steps
  • No proprietary instrument to purchase-runs on any real-time PCR instrument

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; TAU; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)
Assay Range 0.064 to 5000 pg/mL
Assay Sensitivity <0.05 pg/mL
Conjugate FAM™
Product Type ProQuantum
Sample Type Cerebrospinal Fluid, Plasma, Serum, Supernatant
For Use With (Equipment) qPCR Instruments
Gene ID (Entrez) 4137
Gene Symbol MAPT
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Cerebrospinal Fluid 2-5 μL, Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Storage Requirements -20°C
Target Tau
Test Time 30 mins
Total Assay Time 2 hrs
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