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Human TIM-3 Antibody, R&D Systems™
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Catalog No. AF2365SP
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AF2365SP 25 μg
AF2365 100 μg
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Description

Goat Polyclonal Antibody has been used in 16 publications

TIM-3 Polyclonal specifically detects TIM-3 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, CyTOF-ready, Knockout Validated, Dual RNAscope ISH-IHC.

Specifications

Antigen TIM-3
Applications Western Blot, Flow Cytometry, Immunohistochemistry, CyTOF, Immunoassay, In Situ Hybridization (ISH)
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 3-15 ug/mL, CyTOF-ready, Knockout Validated 1 ug/mL, Dual RNAscope ISH-IHC 3-25 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q8TDQ0
Gene Alias CD366, FLJ14428, HAVcr-2, HAVCR2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, KIM-3, SPTCL, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, Tim-3, TIM3, TIM3 T-cell membrane protein 3, TIMD3, TIMD-3, TIMD3KIM-3
Gene Symbols HAVCR2
Host Species Goat
Immunogen Mouse myeloma cell line NS0-derived recombinant human TIM-3 Ser22-Arg200 Accession # Q8TDQ0
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 84868
Test Specificity Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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