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Human TNF-alpha Antibody, R&D Systems™
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Catalog No. MAB610SP
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Catalog No. Quantity
MAB610SP 25 μg
MAB610100 100 μg
MAB610500 500 μg
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Description

Mouse Monoclonal Antibody has been used in 43 publications

TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples. It is validated for Western Blot, Neutralization, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen TNF-alpha
Applications Western Blot, Neutralization, Immunocytochemistry, ELISA
Classification Monoclonal
Clone 28401
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Neutralization 0.01-0.04 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P01375
Gene Alias APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene Symbols TNF
Host Species Mouse
Immunogen E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7124
Test Specificity Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF-beta , recombinant mouse TNF-alpha , recombinant rat TNF-alpha , and recombinant porcine TNF-alpha is observed.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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