Human TNF-alpha Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 6 publications

Supplier:  R&D Systems MAB2101100

Catalog No. MAB2101100

Description

TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples. It is validated for Flow Cytometry, Neutralization, CyTOF-ready.

Specifications

• Antigen: TNF-alpha
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.015-0.06 ug/mL, CyTOF-ready
• Gene Accession No.: P01375
• Gene Symbols: TNF
• Immunogen: E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Neutralization, CyTOF
• Clone: 6401
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Host Species: Mouse
• Purification Method: Protein A or G purified from ascites
• Regulatory Status: RUO
• Gene ID (Entrez): 7124
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG1

For Research Use Only