Gibco™ Human TPO (Thrombopoietin), Animal-Free Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

Specifications

• Accession Number: P40225
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 7066
• Molecular Weight (g/mol): 18.6 kDa
• Name: Human TPO (Thrombopoietin), Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 250 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: C-mpl ligand; Megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; Mgdf; MKCSF; ML; MPL ligand; Mpllg; MSA; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; TDH2A; THCYT1; THPO; Thrombopoietin; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; Tpo; TPX
• Common Name: Thrombopoietin
• Gene Symbol: THPO
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
• Content And Storage: -20°C
• Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.