Invitrogen™ Human TRACP/PAP/ACP5 ELISA Kit

Description

ELISA

Human TRACP/PAP/ACP5 quantitates human TRACP/PAP/ACP5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human TRACP/PAP/ACP5.

Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression is increased in spleen and monocytes of patients with Gaucher's disease; spleenocytes and circulating leukocytes of patients with hairy cell leukemia; spleen of patients with Hodgkin's lymphoma and in the sera of patients undergoing active bone turnover.

Specifications

• Accession Number: acid phosphatase 5, tartrate resistant; acid phosphatase type V; ACP5; HPAP; human purple acid phosphatase; T5ap; Tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5 A; tartrate-resistant acid phosphatase 5 b; tartrate-resistant acid phosphatase type 5; TRACP; TRACP5a; TRACP5b; Trap; TR-AP; TRAPase; TrATPase; TTRRAP; type 5 acid phosphatase
• Assay Range: 0.05 to 10 ng/mL
• Assay Sensitivity: 0.05 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 54
• Gene Symbol: ACP5
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Tartrate Resistant Acid Phosphatase
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov