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Invitrogen™ eBioscience™ Human TSLP ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement of human TSLP protein levels from samples including serum, plasma, and cell culture supernatants.

Manufacturer:  Invitrogen™ 88749786

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Catalog No. 50-174-12


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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
Specifications

Specifications

Q969D9
Biotin
85480
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Human
1 hr 45 min
Human
thymic stromal derived lymphopoietin
8-1,000 pg/mL
ELISA
96-well plates
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
10 x 96 tests
8 pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
TSLP
HRP
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