Gibco™ Human TWEAK, Animal-Free Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Specifications

• Accession Number: O43508
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 8742
• Molecular Weight (g/mol): 17 kDa
• Name: Human TWEAK, Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 5 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: APO3 ligand; APO3/DR3 ligand; APO3L; Dr3l; Dr3lg; LOW QUALITY PROTEIN: tumor necrosis factor ligand superfamily member 12; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4 A; tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; zgc:153941
• Common Name: TWEAK
• Gene Symbol: Tnfsf12
• Biological Activity: Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
• Content And Storage: -20°C
• Activity: Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.