Novus Biologicals™ Human VEGF ELISA Kit (Colorimetric)

ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture. 

Supplier:  Novus Biologicals™ NBP191272

Catalog No. NBP191272

Description

The Human VEGF ELISA Kit (Colorimetric) measures human VEGF in cell culture supernatant, serum, plasma (edta, citrate, heparin).

Specifications

• Assay Range: 15.6 - 1,000pg/mL
• Product Type: ELISA Kit
• Specificity: The assay detects both natural and recombinant human VEGF-A. Cross reactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-A positive serum. There was no crossreactivity detected, notably not with human VEGF-B, VEGF-C, VEGF-D and PfGF. Interference was detected for VEGF-R1 at concentrations > 200 pg/ml, and not for VEGF-R2.
• For Use With (Application): ELISA
• Gene Symbol: VEGFA
• Quantity: 96 Tests
• Storage Requirements: Storage of components varies. See protocol for specific instructions.
• Target: VEGF

• Assay Sensitivity: 7.9pg/mL
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Target Species: Human
• Gene ID (Entrez): 7422
• Kit Contents: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human VEGF-A, Biotin-Conjugate anti-human VEGF-A polyclonal antibody, Streptavidin-HRP, Human VEGF-A Standard lyophilized, 2ng/mL upon reconstitution, Sample Diluent, Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA), Wash Buffer Concentrate 20x (PBS with 1% Tween 20), Substrate Solution (tetramethyl-benzidine), Stop Solution (1M Phosphoric acid), Adhesive Films
• Research Discipline: Angiogenesis, Cancer, Cell Biology, HIF Target Genes, Hypoxia, Signal Transduction
• Synonym: MVCD1, vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, VEGFMGC70609, VPFvascular endothelial growth factor