Novus Biologicals™ Human VEGF ELISA Kit (Colorimetric)

Description

ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture.

The Human VEGF ELISA Kit (Colorimetric) measures human VEGF in cell culture supernatant, serum, plasma (edta, citrate, heparin).

Specifications

• Assay Range: 15.6 - 1,000pg/mL
• Assay Sensitivity: 7.9pg/mL
• Product Type: ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Specificity: The assay detects both natural and recombinant human VEGF-A. Cross reactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-A positive serum. There was no crossreactivity detected, notably not with human VEGF-B, VEGF-C, VEGF-D and PfGF. Interference was detected for VEGF-R1 at concentrations > 200 pg/ml, and not for VEGF-R2.
• Target Species: Human
• For Use With (Application): ELISA
• Gene ID (Entrez): 7422
• Gene Symbol: VEGFA
• Kit Contents: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human VEGF-A, Biotin-Conjugate anti-human VEGF-A polyclonal antibody, Streptavidin-HRP, Human VEGF-A Standard lyophilized, 2ng/mL upon reconstitution, Sample Diluent, Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA), Wash Buffer Concentrate 20x (PBS with 1% Tween 20), Substrate Solution (tetramethyl-benzidine), Stop Solution (1M Phosphoric acid), Adhesive Films
• Quantity: 96 Tests
• Research Discipline: Angiogenesis, Cancer, Cell Biology, HIF Target Genes, Hypoxia, Signal Transduction
• Storage Requirements: Storage of components varies. See protocol for specific instructions.
• Synonym: MVCD1, vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, VEGFMGC70609, VPFvascular endothelial growth factor
• Target: VEGF