Invitrogen™ Human VEGFR3 (Flt-4) Fc Chimera Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (900 μL) be added to the vial to prepare a stock solution of 1.11 mg/mL. Concentration is measured by UV.

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

Specifications

• Accession Number: P35916
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 2324
• Molecular Weight (g/mol): 111.3 kDa
• Name: Human VEGFR3 (Flt-4) Fc Chimera
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AI323512; Chy; chylous ascites; Flt4; FLT-4; FLT41; fms related tyrosine kinase 4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; PCL; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR3; VEGFR-3
• Common Name: VEGF Receptor 3
• Gene Symbol: FLT4
• Biological Activity: Measured by its binding ability in a functional ELISA. 1. Immobilized recombinant human VEGFR3 at 5 μg/ml (100 μL/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/mL 2. Immobilized recombinant human VEGF-C at 10 μg/ml (100 μL/well) can bind recombinant human VEGFR3 (at a linear range of 0.64-80 ng/mL 3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human VEGFR3, amino acids Met1-Ile776 (Accession # NP_002011.2) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. 1. Immobilized recombinant human VEGFR3 at 5 μg/ml (100 μL/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/mL 2. Immobilized recombinant human VEGF-C (at 10 μg/ml (100 μL/well) can bind recombinant human VEGFR3 (at a linear range of 0.64-80 ng/mL 3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.))
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >97% as determined by SDS-PAGE.