R&D Systems™ Human Wnt-3a and Inhibitor IWR Set Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Human Wnt-3a and Inhibitor IWR Set Protein is derived from CHO. The Human Wnt-3a and Inhibitor IWR Set Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 89780
• Quantity: 1 Set
• Source: Chinese Hamster Ovary cell line,CHO-derived human Wnt-3a protein
• Storage Requirements: Store the unopened product at -20 to -70° C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Gene Alias: MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a
• Common Name: Wnt-3a
• Gene Symbol: WNT3A
• Biological Activity: Recombinant Human Wnt-3a induces beta-catenin responsive Topflash activation in HEK293T cells. The specific Wnt pathway inhibitor, endo-IWR 1, inhibits Topflash activity from a 2μg/mL dose of Recombinant Human Wnt-3a with a typical ED50 < 0.2μM. The less active diasteriomer exo-IWR 1 Wnt inhibitor shows minor inhibitory activity in this assay.Recombinant Human Wnt-3a induces alkaline phosphatase production in the MC3T3-E1 mouse pre-osteoblast cell line. The ED50 for this effect is typically 5-25ng/mL. The specific Wnt pathway inhibitor, endo-IWR 1, inhibits alkaline phosphatase production induced from a 50ng/mL dose of Recombinant Human Wnt-3a with an typical ED50 < 0.8μM. The less active diasteriomer exo-IWR 1 Wnt inhibitor shows a > 5μM ED50 with a Recombinant Human Wnt-3a dose of 50ng/ml.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived