Huntingtin, Rabbit anti-Mouse, Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABN90325UG	25 μg

Catalog No. ABN90325UG Supplier MilliporeSigma Supplier No. ABN90325UG

Description

Rabbit Polyclonal Antibody

Anti-Huntingtin, Cat. No. ABN903, is a highly specific rabbit polyclonal antibody that targets Huntington Disease Protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Huntingtin (UniProt: P42858; also known as Huntington disease protein, HD protein) is encoded by the HTT (also known as HD, IT15) gene (Gene ID: 3064) in human. The protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. It is expressed in the brain and is mainly found in the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Huntingtin is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. It plays a role in microtubule-mediated transport and vesicle function. Huntingtin protein contains a nuclear export signal (aa 2395-2404) and 5 HEAT (Huntington, Elongation Factor 3, PR65/A, TOR) domains. Huntingtin protein is cleaved by apopain downstream of the polyglutamine stretch and the resulting N-terminal fragment is cytotoxic and provokes apoptosis. Phosphorylation at Serine 1179 and 1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Mutations in HTT gene cause Huntington disease that is characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Wild-type huntingtin and anti-huntingtin antibody reduces aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of Huntington disease.

Specifications

• Antigen: Huntingtin
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 2 μg/mL from a representative lot detected Huntingtin  in mouse brain tissue lysate.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Huntington disease protein;HD protein
• Gene Symbols: HTT;HD;IT15
• Host Species: Rabbit
• Immunogen: GST/His-tagged recombinant fragment corresponding to 180 amino acids from the N-terminal region of human Huntington disease protein .
• Molecular Weight of Antigen: 347.60 kDa calculated.
• Purification Method: Affinity Purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects Huntington disease protein in human and mouse brain. It targets an epitope within 180 amino acids from the N-terminal region.
• Target Species: Mouse, Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.