Invitrogen™ Hyaluronic acid Polyclonal Antibody

Catalog No.	Quantity
PA185561	1 mL

Catalog No. PA185561 Supplier Invitrogen™ Supplier No. PA185561

Description

Sheep Polyclonal Antibody

For direct ELISA, coat wells with 1% glutaraldehyde for 5 min at room temperature, followed by 3xPBS wash steps prior to coating with hyaluronic acid. Sheep anti hyaluronic acid antibody recognizes hyaluronic acid, a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricating and protective agent.

Hyaluronic acid (HA), is the most abundant glycosaminoglycan (GAG) in mammalian tissue. It is present in high concentrations in connective tissue, such as skin, vitreous humor, cartilage, and umbilical cord, but the largest single reservoir is the synovial fluid (SF) of the diarthrodial joints, where concentrations of 0. 5-4 mg/mL are achieved. Hyaluronic acid, is the major hydrodynamic nonprotein component of joint SF. Its unique viscoelastic properties confer remarkable shock absorbing and lubricating abilities to SF, while its enormous macromolecular size and hydrophilicity serve to retain fluid in the joint cavity during articulation. HA restricts the entry of large plasma proteins and cells into SF but facilitates solute exchange between the synovial capillaries and cartilage and other joint tissues. In addition, HA can form a pericellular coat around cells, interact with proinflammatory mediators, and bind to cell receptors, such as cluster determinant (CD)44 and receptor for hyaluronate-mediated motility (RHAMM), where it modulates cell proliferation, migration, and gene expression. All these physicochemical and biologic properties of HA have been shown to be molecular weight (MW) dependent. The diverse physicochemical properties of HA arise from its unique macromolecular structure. The HA is an exceptionally long (3-30 μm) and unbranched nonsulfated GAG composed of repeating disaccharide units of N-acetylglucosamine, and glucuronic acid glycosidically linked through their respective 1-4 ring positions. Hydroxyl group oxygens at the glucuronyl-1 and glucosamine 3-positions are used for further polymerization of the HA disaccharide units to form chains that, when released from the cell plasma membrane, are of variable length and thus polydispersity. Despite the simplicity of the HA primary structure, this linear polyelectrolyte adopts complex conformations in solution, which engender it with diverse biologic properties. Within the joint cavity, HA molecules are predominately synthesized by the type B synovial cells. (PMID: 12219318). [National Center for Biotechnology Information. PubChem Compound Database; CID=24728612].

Specifications

• Antigen: Hyaluronic acid
• Applications: ELISA
• Classification: Polyclonal
• Concentration: 5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: C28H44N2O23
• Host Species: Sheep
• Immunogen: Hyaluronic acid from human umbilical cord.
• Purification Method: Protein G
• Quantity: 1 mL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG
• Target Molecule: Chemical

Frequently Asked Questions (FAQs)

How should I prepare my plates for a direct ELISA using the Hyaluronic acid Polyclonal Antibody (Cat. No. PA1-85561)?

For direct ELISA, we recommend coating wells with 1% glutaraldehyde for 5 min at room temperature, followed by 3X PBS wash steps before coating with the Hyaluronic acid Polyclonal Antibody (Cat. No. PA1-85561).

What is the molecular weight of the Hyaluronic acid Polyclonal Antibody (Cat. No. PA1-85561)?

The average molecular weight for the Hyaluronic acid Polyclonal Antibody (Cat. No. PA1-85561) is 150 kDa.