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Hygromycin B from Streptomyces Hygroscopicus, MP Biomedicals™

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Description

Hygromycin B is an antibiotic substance isolated from Streptomyces hygroscopicus.

Its mode of action is the inhibition of protein synthesis, by inducing the misreading of the m-RNA template in the prokaryote, (E. coli, at 100 mg/mL), lower eukaryotes (e.g., yeast, at 200mg/mL) and higher eukaryotes (e.g., mammalian cells in culture). It selectively penetrates cells that have been rendered permeable by virus infection and combined with its potency to inhibit translation, it is an effective antiviral agent. Hygromycin B can be used as a dual-selection antibiotic when used in conjunction with G418 or Zeocin™ for cells transfected with two different vectors.

  • Blocks polypeptide synthesis and inhibits elongation. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template.
  • When using this product for mammalian cell selection the working concentration is generally between 50mg/mL and 1mg/mL, but at times may go as high as 1 mg/mL.
  • The appropriate concentration for use must be determined empirically for each cell type.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 31282-04-9
Molecular Formula C20H37N3O13
Molecular Weight (g/mol) 527.524
InChI Key GRRNUXAQVGOGFE-BBMONYMYSA-N
Synonym O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine
PubChem CID 134129613
IUPAC Name (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
SMILES CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N

Specifications

Physical Form Powder
Melting Point 160°C to 180°C (literature)
Quantity 1 g
Solubility Information Freely soluble in water (50 mg/mL), methanol,buffer solution and ethanol. Practically insoluble in less polar solvents.
Formula Weight 527.52g/mol
Percent Purity ∼95%

Safety and Handling

Recommended Storage Store at +4°C.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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