Invitrogen™ HYOU1 Monoclonal Antibody (6E3-2C3), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545339

Catalog No. PIMA545339

Description

1 μg/mL of MA5-45339 was sufficient for detection of GRP170 in 20 μg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 170kDa.

HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.

Specifications

• Antigen: HYOU1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene Accession No.: Q63617, Q9JKR6, Q9Y4L1
• Gene Symbols: HYOU1
• Immunogen: Recombinant Full length GRP170 Protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Western Blot, Immunocytochemistry
• Clone: 6E3-2C3
• Conjugate: FITC
• Gene: HYOU1
• Gene Alias: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; AI415631; Cab140; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; FLJ94899; FLJ97572; Grp 170; Grp170; GRP-170; HSP 12A; HSP12A; HYOU1; hypoxia up-regulated 1; hypoxia up-regulated protein 1; Orp 150; Orp150; ORP-150; oxygen regulated protein (150kD)
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 10525, 12282, 192235
• Content And Storage: 4°C, store in dark
• Form: Liquid