Invitrogen™ HYOU1 Monoclonal Antibody (6E3-2C3)

Catalog No.	Quantity
PIMA527642	100 μg

Catalog No. PIMA527642

Supplier: Invitrogen™ MA527642

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-27642 was sufficient for detection of GRP170 in 20 μg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 170kDa.

HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.

Specifications

• Antigen: HYOU1
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 6E3-2C3
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene: HYOU1
• Gene Accession No.: Q63617, Q9JKR6, Q9Y4L1
• Gene Alias: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; AI415631; Cab140; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; FLJ94899; FLJ97572; Grp 170; Grp170; GRP-170; HSP 12A; HSP12A; HYOU1; hypoxia up-regulated 1; hypoxia up-regulated protein 1; Orp 150; Orp150; ORP-150; oxygen regulated protein (150kD)
• Gene Symbols: HYOU1
• Host Species: Mouse
• Immunogen: Raised against a synthetic peptide of human GRP170.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10525, 12282, 192235
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b