Thermo Scientific™ Hypersil™ PREP BDS C8 HPLC Columns

Catalog No.	Length (Metric)	Diameter (Metric)	Particle Size	Column Format
03-252-897	30 mm	20 mm	10 μm	Guard 1/8 in.
03-252-835	50 mm	2 mm	3 μm	Preparative Column, Standard
03-252-843	50 mm	4.6 mm	3 μm	Preparative Column, Standard
03-252-836	100 mm	2 mm	3 μm	Preparative Column, Standard
03-252-844	100 mm	4.6 mm	3 μm	Preparative Column, Standard
03-252-837	150 mm	2 mm	3 μm	Preparative Column, Standard
03-252-845	150 mm	4.6 mm	3 μm	Preparative Column, Standard
03-252-838	250 mm	2 mm	3 μm	Preparative Column, Standard
03-252-846	250 mm	4.6 mm	3 μm	Preparative Column, Standard
03-252-839	50 mm	2 mm	5 μm	Preparative Column, Standard
03-252-847	50 mm	4.6 mm	5 μm	Preparative Column, Standard
03-252-851	50 mm	10 mm	5 μm	Preparative Column, Standard
03-252-852	50 mm	20 mm	5 μm	Preparative Column, Standard
03-252-880	50 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-252-840	100 mm	2 mm	5 μm	Preparative Column, Standard
03-252-848	100 mm	4.6 mm	5 μm	Preparative Column, Standard
03-252-853	100 mm	10 mm	5 μm	Preparative Column, Standard
03-252-854	100 mm	20 mm	5 μm	Preparative Column, Standard
03-252-881	100 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-252-841	150 mm	2 mm	5 μm	Preparative Column, Standard
03-252-849	150 mm	4.6 mm	5 μm	Preparative Column, Standard
03-252-855	150 mm	10 mm	5 μm	Preparative Column, Standard
03-252-856	150 mm	20 mm	5 μm	Preparative Column, Standard
03-252-882	150 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-252-857	150 mm	30 mm	5 μm	Preparative Column, Standard
03-252-883	150 mm	30 mm	5 μm	Preparative Column, Xtended Life
03-252-858	150 mm	50 mm	5 μm	Preparative Column, Standard
03-252-884	150 mm	50 mm	5 μm	Preparative Column, Xtended Life
03-252-842	250 mm	2 mm	5 μm	Preparative Column, Standard
03-252-850	250 mm	4.6 mm	5 μm	Preparative Column, Standard
03-252-859	250 mm	10 mm	5 μm	Preparative Column, Standard
03-252-860	250 mm	20 mm	5 μm	Preparative Column, Standard
03-252-885	250 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-252-861	250 mm	30 mm	5 μm	Preparative Column, Standard
03-252-886	250 mm	30 mm	5 μm	Preparative Column, Xtended Life
03-252-862	250 mm	50 mm	5 μm	Preparative Column, Standard
03-252-887	250 mm	50 mm	5 μm	Preparative Column, Xtended Life
03-252-875	8 mm	10 mm	10 μm	Guard Column
03-252-876	20 mm	10 mm	10 μm	Guard Column
03-252-878	30 mm	10 mm	10 μm	Guard 1/16 in.
03-252-896	30 mm	10 mm	10 μm	Guard 1/8 in.
03-252-879	30 mm	20 mm	10 μm	Guard 1/16 in.
03-252-863	50 mm	10 mm	10 μm	Preparative Column, Standard
03-252-864	50 mm	20 mm	10 μm	Preparative Column, Standard
03-252-888	50 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-252-865	100 mm	10 mm	10 μm	Preparative Column, Standard
03-252-866	100 mm	20 mm	10 μm	Preparative Column, Standard
03-252-889	100 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-252-867	150 mm	10 mm	10 μm	Preparative Column, Standard
03-252-868	150 mm	20 mm	10 μm	Preparative Column, Standard
03-252-890	150 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-252-869	150 mm	30 mm	10 μm	Preparative Column, Standard
03-252-891	150 mm	30 mm	10 μm	Preparative Column, Xtended Life
03-252-870	150 mm	50 mm	10 μm	Preparative Column, Standard
03-252-892	150 mm	50 mm	10 μm	Preparative Column, Xtended Life
03-252-871	250 mm	10 mm	10 μm	Preparative Column, Standard
03-252-872	250 mm	20 mm	10 μm	Preparative Column, Standard
03-252-893	250 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-252-873	250 mm	30 mm	10 μm	Preparative Column, Standard
03-252-894	250 mm	30 mm	10 μm	Preparative Column, Xtended Life
03-252-874	250 mm	50 mm	10 μm	Preparative Column, Standard
03-252-895	250 mm	50 mm	10 μm	Preparative Column, Xtended Life

Catalog No. 03-252-897 Supplier Thermo Scientific™ Supplier No. 40110029323B

Description

Scale up to prep LC using Hypersil PREP C8 BDS HPLC columns which offers selectivity similar to C18 but with reduced retention in analytical and preparative scale.

Scale up from analytical to a preparative LC method with Thermo Scientific™ Hypersil™ PREP BDS C8 reversed phase preparative HPLC column. Our columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. With similar selectivity to C18 columns and predictable elution order, C8 columns with base deactivation for reduced silanol activity are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations.

Similar selectivity, less retention than C18 columns

• Successful retention and separation of moderately hydrophobic analytes.
• Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns.
• Base deactivated silica.
• C8 (USP L7) selectivity.
• pH stable from 2 to 8.
• Maximum temperature of 60°C.

Faster separations

• Higher analytical throughput compared to C18 alkyl chain chemistry.
• Quicker compound elution due to reduced hydrophobic interactions.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

Specifications

• Column Format: Guard 1/8 in.
• Diameter (Metric): 20 mm
• For Use With: Guard Column Requires Use of Preparative Guard Holder, 891-031
• Length (Metric): 30 mm
• Max. Pressure: 2200 psi (152 bar)
• Particle Size: 10 μm
• pH: 2 to 8
• Pore Size: 145 Å
• Surface Area: 185 m²/g
• Temperature: 60°C
• USP Type: L7
• Stationary Phase: C8
• Packing Material: Spherical, Fully Porous, Base-Deactivated Silica
• Carbon Load: 7%
• Column Type: Reversed Phase
• Endcapped: Yes
• Phase: Reversed Phase