Thermo Scientific™ Hypersil™ PREP HS C8 HPLC Columns

Catalog No.	Length (Metric)	Diameter (Metric)	Particle Size	Column Format
03-252-999	50 mm	2 mm	3 μm	Preparative Column, Standard
03-253-007	50 mm	4.6 mm	3 μm	Preparative Column, Standard
03-253-000	100 mm	2 mm	3 μm	Preparative Column, Standard
03-253-008	100 mm	4.6 mm	3 μm	Preparative Column, Standard
03-253-001	150 mm	2 mm	3 μm	Preparative Column, Standard
03-253-009	150 mm	4.6 mm	3 μm	Preparative Column, Standard
03-253-002	250 mm	2 mm	3 μm	Preparative Column, Standard
03-253-010	250 mm	4.6 mm	3 μm	Preparative Column, Standard
03-253-003	50 mm	2 mm	5 μm	Preparative Column, Standard
03-253-011	50 mm	4.6 mm	5 μm	Preparative Column, Standard
03-253-015	50 mm	10 mm	5 μm	Preparative Column, Standard
03-253-016	50 mm	20 mm	5 μm	Preparative Column, Standard
03-253-044	50 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-253-004	100 mm	2 mm	5 μm	Preparative Column, Standard
03-253-012	100 mm	4.6 mm	5 μm	Preparative Column, Standard
03-253-017	100 mm	10 mm	5 μm	Preparative Column, Standard
03-253-018	100 mm	20 mm	5 μm	Preparative Column, Standard
03-253-045	100 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-253-005	150 mm	2 mm	5 μm	Preparative Column, Standard
03-253-013	150 mm	4.6 mm	5 μm	Preparative Column, Standard
03-253-019	150 mm	10 mm	5 μm	Preparative Column, Standard
03-253-020	150 mm	20 mm	5 μm	Preparative Column, Standard
03-253-046	150 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-253-021	150 mm	30 mm	5 μm	Preparative Column, Standard
03-253-047	150 mm	30 mm	5 μm	Preparative Column, Xtended Life
03-253-022	150 mm	50 mm	5 μm	Preparative Column, Standard
03-253-048	150 mm	50 mm	5 μm	Preparative Column, Xtended Life
03-253-006	250 mm	2 mm	5 μm	Preparative Column, Standard
03-253-014	250 mm	4.6 mm	5 μm	Preparative Column, Standard
03-253-023	250 mm	10 mm	5 μm	Preparative Column, Standard
03-253-024	250 mm	20 mm	5 μm	Preparative Column, Standard
03-253-049	250 mm	25 mm	5 μm	Preparative Column, Xtended Life
03-253-025	250 mm	30 mm	5 μm	Preparative Column, Standard
03-253-050	250 mm	30 mm	5 μm	Preparative Column, Xtended Life
03-253-026	250 mm	50 mm	5 μm	Preparative Column, Standard
03-253-051	250 mm	50 mm	5 μm	Preparative Column, Xtended Life
03-253-039	8 mm	10 mm	10 μm	Guard Column
03-253-040	20 mm	10 mm	10 μm	Guard Column
03-253-042	30 mm	10 mm	10 μm	Guard 1/16 in.
03-253-060	30 mm	10 mm	10 μm	Guard 1/8 in.
03-253-043	30 mm	20 mm	10 μm	Guard 1/16 in.
03-253-061	30 mm	20 mm	10 μm	Guard 1/8 in.
03-253-027	50 mm	10 mm	10 μm	Preparative Column, Standard
03-253-028	50 mm	20 mm	10 μm	Preparative Column, Standard
03-253-052	50 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-253-029	100 mm	10 mm	10 μm	Preparative Column, Standard
03-253-030	100 mm	20 mm	10 μm	Preparative Column, Standard
03-253-053	100 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-253-031	150 mm	10 mm	10 μm	Preparative Column, Standard
03-253-032	150 mm	20 mm	10 μm	Preparative Column, Standard
03-253-054	150 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-253-033	150 mm	30 mm	10 μm	Preparative Column, Standard
03-253-055	150 mm	30 mm	10 μm	Preparative Column, Xtended Life
03-253-034	150 mm	50 mm	10 μm	Preparative Column, Standard
03-253-056	150 mm	50 mm	10 μm	Preparative Column, Xtended Life
03-253-035	250 mm	10 mm	10 μm	Preparative Column, Standard
03-253-036	250 mm	20 mm	10 μm	Preparative Column, Standard
03-253-057	250 mm	25 mm	10 μm	Preparative Column, Xtended Life
03-253-037	250 mm	30 mm	10 μm	Preparative Column, Standard
03-253-058	250 mm	30 mm	10 μm	Preparative Column, Xtended Life
03-253-038	250 mm	50 mm	10 μm	Preparative Column, Standard
03-253-059	250 mm	50 mm	10 μm	Preparative Column, Xtended Life

Catalog No. 03-252-999 Supplier Thermo Scientific™ Supplier No. 41103052130

Description

Use these preparative columns for scale-up purification with high phase loading. They are suitable for all USP-L7 applications.

Get maximum loading for your scale up from analytical to a prep LC method with Thermo Scientific™ Hypersil™ PREP HS C8 reversed phase preparative HPLC column. With similar selectivity to C18 columns and predictable elution order, C8 columns are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. By providing high surface area particles with a 100Å pore size, and a variety of dimensions including an option for XtendedLife hardware, which improves column lifetime by maintaining a uniform packed bed.

Similar selectivity, less retention than C18 columns

• Successful retention and separation of moderately hydrophobic analytes.
• Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns.
• C8 (USP L7) selectivity - high surface area particle with 100Å pore size.
• pH stable from 2 to 8.
• Maximum temperature of 60°C.

Faster separations

• Higher analytical throughput compared to C18 alkyl chain chemistry.
• Quicker compound elution due to reduced hydrophobic interactions.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

Specifications

• Column Format: Preparative Column, Standard
• Diameter (Metric): 2 mm
• Length (Metric): 50 mm
• Max. Pressure: 5800 psi (400 bar)
• Particle Size: 3 μm
• pH: 2 to 8
• Pore Size: 100 Å
• Surface Area: 350 m²/g
• Temperature: 60°C
• USP Type: L7
• Stationary Phase: C8
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Carbon Load: 7%
• Column Type: Reversed Phase
• Endcapped: Yes
• Phase: Reversed Phase