I-A[b] Mouse anti-Mouse, Brilliant Violet 421, Clone: AF6-120.1, BD

Catalog No.	Quantity
BDB566229	25 μg

Catalog No. BDB566229 Supplier BD Biosciences Supplier No. 566229

Description

Mouse Monoclonal Antibody

The AF6-120.1 antibody reacts with the I-A[b] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[k] and H-2[u] haplotypes. Reactivity with other haplotypes (e.g., d, f, g7, p, q, r, s /) has not been observed

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: I-A[b]
• Classification: Monoclonal
• Clone: AF6-120.1
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Gene Alias: Mouse MHC/H2 class II histocompatibility antigen, I-Ab
• Host Species: Mouse
• Immunogen: Mouse C57BL/10J
• Purification Method: Affinity Purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ