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I-A/I-E Rat anti-Mouse, Brilliant Violet 421, Clone: 2G9, BD Optibuild™

Catalog No. BDB743870 Shop All BD Biosciences Products
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BDB743870 50 μg
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Catalog No. BDB743870 Supplier BD Biosciences Supplier No. 743870
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Description

Rat Monoclonal Antibody

The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen I-A/I-E
Classification Monoclonal
Clone 2G9
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias H-2I, Ia Ag, I-Ad, I-Ed, I-Eb, and I-Ek MHC class II alloantigens
Host Species Rat
Immunogen BALB/c mouse epidermal Langerhans cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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