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I-A/I-E Rat anti-Mouse, Brilliant Violet 510, Clone: M5/114.15.2 (also known as M5/114), BD Optibuild™

Catalog No. BDB742893 Shop All BD Biosciences Products
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BDB742893 50 μg
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Catalog No. BDB742893 Supplier BD Biosciences Supplier No. 742893
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Description

Rat Monoclonal Antibody

The M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen I-A/I-E
Classification Monoclonal
Clone M5/114.15.2 (also known as M5/114)
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias M5/114, H-2I, Ia Ag, I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloAgs
Host Species Rat
Immunogen Activated C57BL/6 Mouse Spleen Cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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