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I-A/I-E Rat anti-Mouse, Brilliant Violet 786, Clone: M5/114.15.2 (also known as M5/114), BD Optibuild™

Catalog No. BDB742894 Shop All BD Biosciences Products
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BDB742894 50 μg
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Catalog No. BDB742894 Supplier BD Biosciences Supplier No. 742894
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Description

Rat Monoclonal Antibody

The M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
TRUSTED_SUSTAINABILITY

Specifications

Antigen I-A/I-E
Classification Monoclonal
Clone M5/114.15.2 (also known as M5/114)
Concentration 0.2mg/mL
Conjugate Brilliant Violet 786
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias M5/114, H-2I, Ia Ag, I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloAgs
Host Species Rat
Immunogen Activated C57BL/6 Mouse Spleen Cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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