I-Ap Monoclonal Antibody (7-16.17), FITC, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA517769

Catalog No. PIMA517769

Description

MA5-17769 targets I-Ap in FACS applications and shows reactivity with Mouse samples. The MA5-17769 immunogen is b10.p.

MHC Class II (I-Ap) is a protein complex found on the surface of cells in vertebrates. Its main function is to present peptides derived from extracellular proteins to T cells for immune recognition. MHC Class II (I-Ap) is composed of an alpha and beta chain, both of which contribute to the peptide-binding groove. MHC Class II (I-Ap) is primarily restricted to antigen-presenting cells, including dendritic cells, macrophages, and B cells. These cells are responsible for capturing and processing antigens from the extracellular environment. Once the antigens are internalized, they are broken down into smaller peptides, which then bind to MHC Class II molecules within the endosomal compartments of these cells. MHC Class II (I-Ap) consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for interacting with the antigenic peptides, while the transmembrane domain anchors the MHC Class II molecule to the cell membrane. The cytoplasmic tail plays a role in intracellular signaling events. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: I-Ap
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 4-5mg/mL BSA and 0.02% sodium azide
• Gene Accession No.: P04230
• Gene Symbols: H2-Eb1
• Immunogen: B10.p
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry
• Clone: 7-16.17
• Conjugate: FITC
• Gene: H2-Eb1
• Gene Alias: cell surface glycoprotein; H-2 class II histocompatibility antigen, I-E beta chain; H2Eb; H-2Eb; H2-Eb1; histocompatibility 2, class II antigen E beta; Ia4; Ia-4; I-E beta MHC class II; major histocompatibility complex H-2E beta chain; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 14969
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Form: Liquid