Molecular Probes™ BacLight™ Bacterial Membrane Potential Kit

Description

BacLight™ Bacterial Membrane Potential Kit

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC₂(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC₂(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

Specifications

• Content And Storage: Contains DiOC₂(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Membrane Potential Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Bacterial Membrane Potential Kit
• Dye Type: Membrane Potential Probes
• Emission: 482/497
• Format: Tube(s)
• Product Line: BacLight
• Quantity: 1 kit
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.