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Molecular Probes™ BacLight™ Green Bacterial Stain

Catalog No. B35000
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B35000 20 x 50 μg
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Catalog No. B35000 Supplier Molecular Probes™ Supplier No. B35000
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Description

BacLight™ Green Bacterial Stain

The BacLight Green (Cat. no. B-35000) and BacLight Red (Cat. no. B-35001) bacterial stains are fluorescent labeling reagents for detecting and monitoring bacteria. These two dyes are not nucleic acid stains. Bacteria stained with the BacLight Green and BacLight Red bacterial stains exhibit bright green and red fluorescence (absorption/emission ∼480/516 and ∼581/644 nm, respectively), and can be resolved using the appropriate flow cytometric channels or fluorescence microscopy filters. The BacLight bacterial stains are compatible with formaldehyde or alcohol fixation methods.

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Bacteria
Content And Storage Contains 20 vials of BacLight™ green bacterial stain (50 ug per vial). Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Bacterial Stain
Dye Type Other Label(s) or Dye(s)
Emission 480/ 561
Form Solid
Format Tube(s), Slide(s)
Product Line BacLight
Quantity 20 x 50 μg
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
What optical filter do your recommend with the BacLight Green Bacterial Stain?

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

For Research Use Only. Not for use in diagnostic procedures.

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