Invitrogen™ iBright™ Prestained Protein Ladder

Catalog No.	Quantity
LC5615	2 x 250 μL
LC5615X4	8 x 250 μL

Catalog No. LC5615 Supplier Invitrogen™ Supplier No. LC5615

Description

For visualization and protein sizing during Electrophoresis and confirmation of Western blot detection.

Invitrogen iBright Prestained Protein Ladder provide direct visualization during electrophoresis and transfer of Western blots, and chemiluminescent or fluorescent Western blot visualization of two unstained proteins for confirmation of western blot detection.

The iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two proteins (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. These two unstained proteins are compatible with chemiluminescent substrates and fluorescent antibodies.

• Broad size range: twelve proteins spanning 11 to 250 kDa
• Fluorescent visualization: detect the ten stained proteins using the 670nm red laser or 700nm channel of a fluorescence imager
• Western blot confirmation: detect the two unstained proteins using the detection system for the target protein
• Ready to use: supplied in a loading buffer for direct loading on gels
• Sharp bands: clear, sharp bands for direct visualization on both gel and membrane
• Reference band: 55 kDa band has greater intensity for easy orientation
• Quality tested: each lot evaluated by SDS-PAGE and western blotting

Warranty and Services

1 year

Specifications

• Content And Storage: Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol
  
  Upon receipt, store at –20°C. Product is shipped on ice.
• Detection Method: Colorimetric, NIR Fluorescence, and User defined detection system (2 bands)
• Number of Markers: 12
• Ready to Load: Yes
• Size Range: 11 to 250 kDa
• Gel Compatibility: Novex™ Midi Gels, Novex™ Mini Gels, Novex™Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Gels, NuPAGE™ MES Gels, NuPAGE™ MOPS Gels, NuPAGE™ Tris-Acetate Gels, Precise™ Tris-Glycine Gels, SDS-PAGE Gels, Precise™ Tris-HEPES Gels
• Molecular Weight (g/mol): 250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa
• Quantity: 2 x 250 μL
• Shipping Condition: Dry Ice
• Product Line: iBright
• Product Type: Protein Ladder
• Stain Type: Blue
• System Type: Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.