Invitrogen™ ICAD Recombinant Rabbit Monoclonal Antibody (JE49-23)

Catalog No.	Quantity
PIMA542485	100 μL

Catalog No. PIMA542485 Supplier Invitrogen™ Supplier No. MA542485

Description

Rabbit Recombinant Monoclonal Antibody

Positive Control: Jurkat cell lysate, human stomach tissue lysate, human thyroid tissue, human breast tissue, human small intestine tissue, human pancreas intestine tissue, HCT116. Subcellular Location: Cytoplasm.

ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.

Specifications

• Antigen: ICAD
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: JE49-23
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene: DFFA
• Gene Accession No.: O00273
• Gene Alias: A330085O09Rik; DFF; DFF1; DFF35; Dff45; DFF-45; Dffa; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; H13; ICAD; ICAD-L; ICAD-S; Inhibitor of CAD; RP23-121D17.3
• Gene Symbols: DFFA
• Host Species: Rabbit
• Immunogen: Recombinant protein within human ICAD aa 37-137.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1676
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG