Novus Biologicals™ ICAM-1/CD54 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse ICAM-1/CD54.

Specifications

• Assay Range: 8.75 pg/mL
• Assay Sensitivity: 8.75-560 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse ICAM-1/CD54.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: BB2, CD54, CD54 antigen, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor, P3.58
• Gene ID (Entrez): 3383
• Gene Symbol: ICAM1
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 20 ng of recombinant Mouse ICAM-1/CD54.
  Capture Antibody: 1 mg/mL of rabbit anti-Mouse ICAM-1/CD54 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-ICAM-1/CD54 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Immunology, Mesenchymal Stem Cell Markers, Myeloid derived Suppressor Cell, Phospho Specific, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: ICAM-1/CD54
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 560 pg/mL is recommended.