ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24433100	Novus Biologicals NBP2443310.02MG	0.02 mg
NBP24433101	Novus Biologicals NBP2443310.1MG	0.1 mg
NBP24433102	Novus Biologicals NBP2443310.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ICAM-1/CD54 Monoclonal specifically detects ICAM-1/CD54 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Functional, Immunofluorescence.

Specifications

• Antigen: ICAM-1/CD54
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cancer, Cell Biology, Immunology, Mesenchymal Stem Cell Markers, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 3383
• Immunogen: Raji Burkitt lymphoma cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: W-CAM-1 (same as Wehi-CAM-1 or 1H4)
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: BB2, CD54, CD54 antigen, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor, P3.58
• Gene Symbols: ICAM1
• Isotype: IgG2b κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

For Research Use Only