MilliporeSigma™ ICOS/CD278, Armenian Hamster, Unlabeled, Clone: C398.4A,

Catalog No.	Quantity	Conjugate
MABF977MI	100 μg	Unconjugated

Catalog No. MABF977MI Supplier MilliporeSigma™ Supplier No. MABF977

Description

Armenian Hamster Monoclonal Antibody

Inducible T-cell costimulator (UniProt Q9WVS0; also known as Activation-inducible lymphocyte immunomediatory molecule, CCLP, CD278, CD28 and CTLA-4-like protein, CD28-related protein 1, CRP-1, H4) is encoded by the Icos (also known as Ailim) gene (Gene ID 54167) in murine species. ICOS is a costimulatory molecule expressed on activated T cells and regulatory T cells, it plays a crucial role in T-cell survival and function, Th2 cell differentiation, and lung inflammatory responses. Upon ICOS-ligand (ICOS-L) binding, ICOS initiates a cascade of intracellular signaling events, leading to the production of cytokines such as IL-4 and IL-13. Both ICOS and ICOS-L are expressed on type 2 innate lymphoid cells (ILC2s) that, along with Th2 cells, contribute to the pathogenesis of asthma by producing copious amounts of IL-5 and IL-13. ICOS and ICOS-L interaction is reported to promote cytokine production and survival of ILC2s through STAT5 signaling. ICOS-deficiency or blocking ICOS and ICOS-L interaction is shown to prevent cytokine-induced asthma and lung inflammation in a humanized murine model with adoptively transferred human peripheral ILC2s. Human and murine ICOS proteins are produced with a signal peptide (a.a. 1-20) sequence, which is removed posttranslationally to yield the mature protein with a large N-terminal extracellular (human a.a. 21-140 and murine a.a. 21-144) region composed almost entirely of a V-type Ig-like domain (human a.a. 30-132 and murine a.a. 30-133), followed by a transmembrane domain (human a.a. 141-161 and murine a.a. 145-165) and a cytoplasmic tail (human a.a. 162-199 and murine a.a. 166-200).

Specifications

• Antigen: ICOS/CD278
• Applications: Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Classification: Monoclonal
• Clone: C398.4A
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified Armenian hamster monoclonal antibody IgG in PBS without preservatives.
• Gene: Icos, Ailim
• Gene Accession No.: Q9WVS0
• Host Species: Armenian Hamster
• Immunogen: D10.G4.1 murine TH2 cells (Redoglia, V., et al. (1996). Eur. J. Immunol. 26(11):2781-2789).
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation & Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_059508
• Test Specificity: Clone C398.4A recognizes a extracellular epitope present in murine (UniProt Q9WVS0) and the two alternatively spliced human ICOS/CD278 isoforms (UniProt Q9Y6W8).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG

For Research Use Only. Not for use in diagnostic procedures.