Invitrogen™ ID3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5100268

Catalog No. PIPA5100268

Description

Antibody detects endogenous levels of total ID3.

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Specifications

• Antigen: ID3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Id3
• Gene Alias: 1R21; bHLHb25; class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; HEIR-1; Helix-loop-helix protein HEIR-1; Hlh462; HLH642; ID 3; Id3; Id-3; Idb3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of DNA binding 3, HLH protein
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15903, 25585, 3399
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P41133, P41138, Q02535
• Gene Symbols: Id3
• Immunogen: A synthesized peptide derived from human ID3(Accession Q02535), corresponding to amino acid residues S21-Q71.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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