Invitrogen™ IDH1 (R132H) Recombinant Rabbit Monoclonal Antibody (RM390)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA544585

Catalog No. FEMA544585

Description

IDH1 (R132H) Recombinant Monoclonal Antibody for IHC (P)

Isocitrate dehydrogenases such as IDH1 catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. This product is specific for IDH1 (mutant R132H).

Specifications

• Antigen: IDH1 (R132H)
• Classification: Recombinant Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene Accession No.: O75874
• Gene Symbols: IDH1
• Immunogen: A peptide corresponding to human (R132H mutant specific). The immunogen range is a synthetic peptide within amino acids 180-280 of human IDH1 with R132H mutant.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: RM390
• Conjugate: Unconjugated
• Gene: IDH1
• Gene Alias: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDH1-mutant; IDP; IDPC; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; oxalosuccinate decarboxylase; PICD
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3417
• Content And Storage: -20°C
• Form: Liquid