Invitrogen™ IDH2 Monoclonal Antibody (6B13)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549159

Catalog No. PIMA549159

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Immunogen sequence identical to the related mouse and rat sequences. Positive Control - WB: human Hela whole cell, human Sw620 whole cell, human MCF-7 whole cell, human HEPG2 whole cell, human Jurkat whole cell, rat heart tissue, rat liver tissue, rat PC-12 whole cell, mouse heart tissue, mouse liver tissue, mouse NIH/3T3 whole cell. IHC: human testicular cancer tissue, human rectal cancer tissue, human melanoma tissue, human ovarian cancer tissue, rat skeletal muscle tissue. ICC/IF: A431 cell. Flow: SiHa cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Specifications

• Antigen: IDH2
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P48735, P54071, P56574
• Gene Symbols: Idh2
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 6B13
• Conjugate: Unconjugated
• Gene: Idh2
• Gene Alias: D2HGA2; E430004F23; ICD-M; IDH; Idh2; Idh-2; IDHM; IDP; IDPM; isocitrate dehydrogenase (NADP(+)) 2; isocitrate dehydrogenase (NADP(+)) 2, mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; NADPH-specific isocitrate dehydrogenase; oxalosuccinate decarboxylase
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 269951, 3418, 361596
• Content And Storage: -20°C
• Form: Lyophilized