Invitrogen™ IFI16 Monoclonal Antibody (2I3D7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549174

Catalog No. PIMA549174

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Daudi whole cell, human Raji whole cell, human MOLT-4 whole cell, human U-87 MG whole cell. ICC/IF: A431 cell. Flow: U251 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Specifications

• Antigen: IFI16
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q16666
• Gene Symbols: IFI16
• Immunogen: E.coli-derived human IFI16 recombinant protein (Position: E183-K743).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 2I3D7
• Conjugate: Unconjugated
• Gene: IFI16
• Gene Alias: gamma-interferon-inducible protein 16; IFI 16C; IFI16; Ifi-16; IFNGIP1; interferon gamma inducible protein 16; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; interferon-inducible myeloid differentiation transcriptional activator; PYHIN2
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3428
• Content And Storage: -20°C
• Form: Lyophilized