IFN-alpha 2 Antibody (N39), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24509300	Novus Biologicals NBP2450930.02MG	0.02 mg
NBP24509301	Novus Biologicals NBP2450930.1MG	0.1 mg
NBP24509302	Novus Biologicals NBP2450930.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

IFN-alpha 2 Monoclonal specifically detects IFN-alpha 2 in Human samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence.

Specifications

• Antigen: IFN-alpha 2
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cytokine Research
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 3440
• Immunogen: Purified recombinant human IFN-alpha2
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: N39
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: alpha-2a interferon, IFNA, IFN-alpha-2, IFN-alphaA, INFA2, interferon alpha 2b, interferon alpha A, interferon alpha-2, Interferon alpha-A, interferon, alpha 2, LeIF A, MGC125764, MGC125765
• Gene Symbols: IFNA2
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this MAb is called site I and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 MAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.

For Research Use Only