Invitrogen™ Human IFN beta ELISA Kit

Description

ELISA

The Human Interferon beta (Hu IFNβ) ELISA quantitates Hu IFNβ in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNβ. Principle of the method The Human IFNβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

Specifications

• Accession Number: P01574
• Assay Range: 50 to 4000 pg/mL
• Assay Sensitivity: 50 pg/mL
• Description: Human IFN beta ELISA Kit
• Conjugate: HRP
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Product Type: ELISA
• Sample Type: Supernatant
• Specificity: No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß
• Sufficient For: 96 microplate wells
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3456
• Gene Symbol: IFB,IFF,IFN-beta,IFNB
• Interassay CV: <8%
• Intraassay CV: <8
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody Concentrate, Anti-IgG-HRP Concentrate, Concentrate Diluent, Standard, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Measurement: Absorbance at 450 nm
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IFN beta
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 15 mins

Safety and Handling

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