Invitrogen™ IFN beta Human ELISA Kit
Measure levels of human interferon beta (IFNb) in human cell and tissue culture media supernatant samples using 96-well plates.
Supplier: Invitrogen™ 414101
Assay IFN-b (human interferon beta) levels in human cell and tissue culture media (supernatants) with theseInvitrgen™ Human IFN beta ELISA Kits.Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
|No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß|
|Pre-coated 96 well Microplate, Detection Antibody Concentrate, Anti-IgG-HRP Concentrate, Concentrate Diluent, Standard, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Adhesive Plate Covers|
|Absorbance at 450nm|
|Supernatant, 50 μL|
|3 hr. 15 min.|
|Human IFN beta ELISA Kit|
|Colorimetric ELISA (TMB substrate) in clear 96-well plate|
|96 microplate wells|
|Colorimetric Microplate Reader|
|Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%|
|2°C to 8°C|
|1 hr. 20 min.|
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