Invitrogen™ IFN beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P32BBT50UG

Catalog No. 500P32BBT50

Description

AA Sequence of recombinant protein: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-beta. Anti-Human IFN-beta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IFN-beta (500-P32B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta. Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P32BBT-1MG will be provided as 2 x 500 μg

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

Specifications

• Antigen: IFN beta
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Ifnb1
• Gene Alias: Fibroblast interferon; Ifb; IFF; IFN b; IFN ß; IFNB; Ifnb1; IFNbeta; IFN-beta; IFNß; Interferon; Interferon b; Interferon beta; interferon beta 1; interferon beta 1, fibroblast; interferon beta protein; interferon, beta 1; interferon, beta 1, fibroblast; interferon-beta; RP11-113D19.1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3456
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P01574
• Gene Symbols: Ifnb1
• Immunogen: E.coli-derived Recombinant Human IFN-beta.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody