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PeproTech IFN beta Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P32B100
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500P32B100 100 μg
500P32B50UG 50 μg
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Catalog No. 500P32B100 Supplier PeproTech Supplier No. 500P32B100UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-beta. Anti-Human IFN-beta-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-beta matrix. Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-beta (500-P32BBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta. Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IFN beta
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Ifnb1
Gene Accession No. P01574
Gene Alias Fibroblast interferon; Ifb; IFF; IFN b; IFN β; IFNB; Ifnb1; IFNbeta; IFN-beta; IFNβ; Interferon; Interferon b; Interferon beta; interferon beta 1; interferon beta 1, fibroblast; interferon beta protein; interferon, beta 1; interferon, beta 1, fibroblast; interferon-beta; RP11-113D19.1
Gene Symbols Ifnb1
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human IFN-beta.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3456
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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