Novus Biologicals™ IFN-gamma Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IFN-gamma.

Specifications

• Assay Range: 10.94 pg/mL
• Assay Sensitivity: 10.94-700 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IFN-gamma.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: IFG, IFI, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
• Gene ID (Entrez): 3458
• Gene Symbol: IFNG
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human IFN-gamma monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Human IFN-gamma monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 40 ng of recombinant Human IFN-gamma.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: IFN-gamma
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. 3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 700 pg/mL is recommended.