Invitrogen™ Rat IFN gamma ELISA Kit

Description

ELISA

The Rat Interferon gamma (Rt IFNγ) ELISA quantitates Rt IFNγ in rat serum,plasma,or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IFNγ. Principle of the method The Rat IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01581
• Assay Range: 8 to 500 pg/mL
• Assay Sensitivity: <2 pg/mL
• Description: IFN gamma ELISA Kit, 1 Kit, 480 Assays, 50μL Sample Size, 8 to 500pg/mL Assay Range, 2.5 hrs. Assay Time, Rat Species, IFNγ Target, HRP Conjugate, TMB Substrate, < 2pg/mL Sensitivity, Colorimetric Detection Method, Store kit at 2 to 8°C, Used fo
• Assay: 480
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: 96-well Strip Plate
• Product Type: ELISA
• Protein Family: Cytokines and receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: No significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES or TNFα or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250 pg/mL mouse IFNγ was equ
• Substrate Type: TMB
• Sufficient For: 480 Assay
• Target Species: Rat
• For Use With (Application): Protein Assays and Analysis, Protein Biology, ELISA, Ready-to-Use Immunoassay
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 25712
• Gene Symbol: Ifng
• Host Species: Rat
• Interassay CV: 8.7%
• Intraassay CV: 8.5
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Measurement: Absorbance at 450 nm minus absorbance (at 550 nm)
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IFN gamma
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins